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EPIC CODE: LAB5171 Testosterone, Free and Total (Includes Sex Hormone Binding Globulin)

ADDITIONAL TEST CODE

Sunquest: TESFTL

REGIONAL HOSPITAL PERFORMING

UPH Methodist Hospital
UPH Pekin and UPH Proctor Hospitals will transport to UPH Methodist for testing.

Collect

One Gold, Red or Green top tube (min: 6 mL)

Storage/Transport Temperature

Refrigerated

Stability (from collection to initiation)

After separation from cells: Ambient: 4 hours; Refrigerated: 48 hours; Frozen (-20o C) Stability: 1 month

Unacceptable Conditions

Grossly hemolyzed

Days Performed

Daily

Methodology

Quantitative Electrochemiluminescent Immunoassay
The concentration of free testosterone is derived from a mathematical expression based on the constant for the binding of testosterone to sex hormone binding globulin.

Reference Interval

Pediatric Males and Female by age:

MALES ng/dL FEMALES ng/dL
2-10 <8 – 31 2-10 <8 – 80
11 <8-321 11- 15 <8 – 49
12 <8-531 16 – 17 20-56
13 <8 – 609
14 23-652
16 – 17 116-779

Testosterone – Pediatric males and females by Tanner Stage

TANNER TANNER
Males ng/dL Females ng/dL
Tanner Stage 1 <8 – 64 Tanner Stage 1 <8 – 79
Tanner Stage 2 <8 – 166 Tanner Stage 2 <8 – 45
Tanner Stage 3 <8 – 609 Tanner Stage 3 <8 – 49
Tanner Stage 4 43-756 Tanner Stage 4 8 – 54
Tanner Stage 5 66 – 841 Tanner Stage 5 14 – 71

Adult Male Testosterone (ng/dL)

18 – 39 years 264 – 916
40 – 49 years 232 – 928
50 – 59 years 210 – 962
60 – 69 years 234 – 990
70 – 79 years 169 – 895

Adult Female Testosterone (ng/dL)

Pre-menopausal 9 – 53
Post – menopausal < 8 – 48

Adult Male SHBG (nmol/L)

17- 66

Adult Female SHBG (nmol/L)

Pre-menopausal: 28 – 146
Post – menopausal 12 – 166

Male Free Testosterone (pg/mL)

50 – 210

Male Free Testosterone (%)

1.6 – 2.9

Adult Female Free Testosterone (pg/mL)

1.0 – 8.5

Adult Female Free Testosterone (pg/mL)

1.0 – 8.5

% Free Testosterone – Pediatric males and females and adult females:

No Reference range has been established for these age groups fo this Free Testosterone Assay.

Interpretive Data

The concentration of free testosterone is derived from a mathematical expression based on the constant for the binding of testosterone to sex hormone binding globulin.

CPT Codes

84402; 84403; 84270

Notes

Please refer to individual components for stability of sample for this test. For testing on children under the age of 2 refer to Testosterone, Free & Total, (Includes Sex Hormone Binding Globulin), Females and Children (0081056).