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EPIC CODE: LAB481 Methotrexate

ADDITIONAL TEST CODE

Sunquest: MTREXO

REGIONAL HOSPITAL PERFORMING

OSF

Collect

One plain red (Min: 3 mL) Protect from Light.
Avoid SST tubes

Storage/Transport Temperature

Plain red or 2 mL serum. (Min: 1 mL) at 2-8 degrees Specimen must be centrifuged and the serum separated within 2 hours of collection. Protect from light.

Stability (from collection to initiation)

Once seperated from cells and protected from light

Ambient (room temperature) Stability: not acceptable

Refrigerated (2-8oC) Stability:(2-8oC) Stability: 14 days

Frozen (<-20oC) Stability: 28 days

Special Testing Notes

Protect from light. Separate serum into an amber plastic vial to protect from light. Draw Specimen 24, 48, or 72 hours after last dose

Remarks

Protect from light.

Days Performed

Referral – OSF

Methodology

Fluorescence Polarization Immunoassay

Additional Information

Methotrexate is an antimetabolite used in the treatment of certain neoplastic diseases, psoriasis and rheumatoid arthritis. In combination with other chemotherapeutic agents, high dose methotrexate doses followed by leucovorin rescue may be effective in prolonging relapse-free survival in patients with non-metastatic osteosarcoma. Methotrexate levels are monitored to assess toxicity.