Sign in →

EPIC CODE: LAB2820 QuantiFERON - TB Gold Plus, 4 tube

Important Note

COLLECTION TIP:  When the tube is upright, blood must meet the small black mark on the label for each tube.   Remember if you use a butterfly you need to have a waste tube to get the air out of the butterfly tubing.

COLLECTING FACILITY: Send to Methodist lab at ROOM TEMPERATURE. 

Additional Codes

Sunquest:  QFT4
ARUP:      3000399

Previously:  Mayo QFT4

REGIONAL HOSPITAL PERFORMING

Collection and Processing performed at UnityPoint Health Methodist, Proctor and Pekin Laboratories

Testing Performed:  ARUP Laboratories

Specimen Preparation

Transport plasma in the original containers. (Min: 0.8 mL per container)

Ordering Recommendations

Aid in the detection of latent disease among persons at increased risk for tuberculosis (TB). Positive predictive value is less in low-risk populations. May be used in persons who have received BCG vaccine.

COLLECTION/TRANSPORT

If specimen is sent to Methodist lab prior to incubation and centrifugation: send samples at AMBIENT temperature. 

After incubation/centrifugation: send to laboratory refrigerated. 

Collect

QuantiFERON-TB Gold Plus (Standard) 4-Tube Collection Kit (ARUP Supply #54012)

or QuantiFERON-TB Gold Plus (HIGH ALTITUDE) 4-Tube Collection Kit (ARUP Supply #54010)

Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. Specimens may remain ambient for up to 16 hours after collection before being placed in an incubator. For collection and transport instructions refer to QuantiFERON under Special Handling at https://aruplab.com/testing/specimen/quantiferon.

Pediatric Requirements

-

Storage/Transport Temperature

Transport storage temperature to Methodist lab: Ambient 

Transport storage temperature to referral lab: Refrigerated.

Specimens may remain ambient for up to 16 hours after collection before being placed in an incubator. 

Stability (after incubation)

Ambient: 2 hours

Refrigerated: 1 month

Frozen: Unacceptable

Unacceptable Conditions

Whole blood

Special Testing Notes

-

Remarks

-

ORDERING

-

Day(s) Performed

Sun-Sat

Duration of Testing

1-2 days

Methodology

Cell Culture/Semi-Quantitative Enzyme-Linked Immunosorbent Assay

COMPONENTS

QuantiFERON Mitogen minus NIL
QuantiFERON NIL
Quantiferon TB Gold Plus
Quantiferon Plus TB1 minus NIL
Quantiferon Plus TB2 minus NIL

Reference Interval

Components Reference Interval
QuantiFERON-TB Gold In-Tube Negative
QuantiFERON-TB1 minus NIL 0.34 IU/mL or less
QuantiFERON-TB2 minus NIL 0.34 IU/mL or less
QuantiFERON MITOGEN minus NIL                    No reference interval        
QuantiFERON NIL No reference interval

Interpretive Data

Interferon gamma release is measured for specimens from each of the four collection tubes. A qualitative result (Negative, Positive, or Indeterminate) is based on interpretation of the four values, NIL, MITOGEN minus NIL (MITOGEN-NIL), TB1 minus NIL (TB1-NIL), and TB2 minus NIL (TB2-NIL). The NIL value represents nonspecific reactivity produced by the patient specimen. The MITOGEN-NIL value serves as the positive control for the patient specimen, demonstrating successful lymphocyte activity. The TB1-NIL tube specifically detects CD4+ lymphocyte reactivity, specifically stimulated by the TB1 antigens. The TB2-NIL tube detects both CD4+ and CD8+ lymphocyte reactivity, stimulated by TB2 antigens. An overall Negative result does not completely rule out TB infection.

A false-positive result in the absence of other clinical evidence of TB infection is not uncommon. Refer to: Updated Guidelines for Using Interferon Gamma Release Assays to Detect Mycobacterium tuberculosis Infection --- United States, 2010 (<https://www.cdc.gov/mmwr/preview/mmwrhtml/rr5905a1.htm>), for more information concerning test performance in low-prevalence populations and use in occupational screening.

CPT

86480