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EPIC CODE: LAB2571 Lactose Tolerance


Sunquest: LACTOV


UPH Methodist Hospital
UPH Pekin Hospital
UPH Proctor Hospital


Methodist Staff: Prior to collection: Call Pharmacy prior to collection to arrange preparation of lactose solution. Adults receive 50g of Lactose in 8 ounces (250 mL) of water.If a child, please communicate patient body weight for calculation of correct dose.

Proctor/Pekin: Call Lab 24 hours prior to collection to arrange preparation of the Lactose solution Adults receive 50g of Lactose. If a child, please have the patient weight for calculation of correct dose.


Patient must be fasting for 8 hours. No eating or drinking during the 3 hour test.


Draw gray top tube for baseline (fasting).

Patient drinks lactose, then draw gray top tubes at 30 min, 60 min, 120 min and 180 min after lactose load. (Min: 3 mL for each sample).

Label each sample with actual time of collection.

Other Acceptable Specimen(s)

Gold top/green top, or 1 mL of serum/plasma (Min: 0.3 mL) at 2-8 C

Seperate serum or plasma from cells ASAP or within 2 hours of collection.

Storage/Transport Temperature

Gray Ambient (room temperature) or 2-8° C. Centrifuged gold top or green top, or 1 mL serum/plasma (Min: 0.3 mL) at or 2-8° C.

seperate serum or plasma from cells ASAP or within 2 hours of collection.

Stability (from collection to initiation)

Stability: (from collection to initiation);

Ambient (room temperature):8 hours; (gray only)

After separation from cells:

Refrigerated: 72 hours; Frozen (-20o C) Stability: 1 year


Sulfasalazine and Sulfapyirdine can interfere with the chemistry analyzers used in the UnityPoint Health Methodist|Proctor Laboratories. Due to the Assay use of NADH/NADPH, when patients are on these medication, the test can have either false elevation or depression of results. Patient’s labs need to be drawn prior to administration of these medications.

Days Performed

Sunday – Saturday



Interpretive Data

A <26 mg/dL increase in glucose over the fasting level, with gastrointestinal symptoms after a lactose load is considered abnormal and consistent with lactase deficiency.

CPT Codes

82951 Tolerance test (GTT); 82952 x2 Tolerance test, each additional beyond three specimens.


if severe lactose deficiency is suspected, the dose should be lowered.