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EPIC CODE: 27 Sezary Diagnostic Flow Cytometry, Blood

ADDITIONAL TEST CODE

MISCLB

REGIONAL HOSPITAL PERFORMING

UnityPoint Health will send sezary cell testing DIRECTLY TO Mayo Medical Laboratory

PREP PRIOR TO COLLECTION

1. Do not transfer blood to other containers.
2. Label specimen as blood

Collect

Monday – Thursday prior to 1300
Peripheral blood: 6 mL EDTA whole blood (Yellow top ACD and green top sodium heparin are also acceptable)

Pediatric Requirements

Peripheral blood: 1-2 mL EDTA whole blood

Storage/Transport Temperature

Ambient (room temperature): Specimens should be received within 72 hours of collection. Testing must be performed within 72 hours of collection.

Stability (from collection to initiation)

Room Temperature is preferred (Refrigerated (2-8oC) Stability:acceptable); 72 hours

Frozen (-20o C) Stability: Unacceptable

Unacceptable Conditions

Frozen; Specimen greater than 72 hours; gross hemolysis

Special Testing Notes

Specimens must be received in Lab prior to 1300 on Thursdays in order to reach destination resting site prior to viability expiration. If special request, contract lab prior to collection.

All sezary cell flow testing will be referred to Mayo Medical Laboratory and sent directly to Mayo Medical Laboratories by UnityPoint Health Methodist.

Remarks

This Sezary panel is ordered in cases of suspected Sezary syndrome or cutaneous T-cell lymphoma (CTCL) with peripheral blood involvement. For cases without a previously confirmed diagnosis of Sezary syndrome, a triage panel will also be performed to exclude a B-cell lymphoproliferative disorder. If there is a significant phenotypically distinct T-cell population detected, a V-beta panel for proof of clonality may be ordered by the signing pathologist.
A triage panel is always performed. The panel is charged based on number of markers tested (FIRST for first marker, ADD1 for each additional marker). In addition, reflex testing may occur to fully characterize a disease state or clarify any abnormalities from the screening test. Reflex tests will be performed at an additional charge for each marker tested (ADD1 if applicable)

Methodology

Flow Cytometry

Components

Flow Cytometry; first cell surface, cytoplasmic or nuclear marker x 1

Flow Cytometry; additional cell surface, cytoplasmic or nuclear marker (each)

Flow Cytometry Interfprattion, 9 to 15 markers (if appropriate)

Flow Cytometry Interpreation, 16 or more markers (if appropriate)

9 markers for sezary

CPT Codes

88184 – Flow Cytometry, first cell surface, cytoplasmic or nucldar marker x 1

88185 – Flow Cytometry, additional cell surface, cytoplasmic or nuclear marker (each)

88188 – Flow Cytometry Interpretation, 9 to 15 markers (if appropriate)

88189 – Flow Cytometry Interpreation, 16 or more markers (if appropriate)